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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2006/06 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Modulation of Chlamydomonas reinhardtii flagellar motility by redox poise |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF CELL BIOLOGY |
| 出版社・発行元 | ROCKEFELLER UNIV PRESS |
| 巻・号・頁 | 173(5),pp.743-754 |
| 著者・共著者 | K Wakabayashi,SM King |
| 概要 | Redox-based regulatory systems are essential for many cellular activities. Chlamydomonas reinhardtii exhibits alterations in motile behavior in response to different light conditions (photokinesis). We hypothesized that photokinesis is signaled by variations in cytoplasmic redox poise resulting from changes in chloroplast activity. We found that this effect requires photosystem I, which generates reduced NADPH. We also observed that photokinetic changes in beat frequency and duration of the photophobic response could be obtained by altering oxidative/reductive stress. Analysis of reactivated cell models revealed that this redox poise effect is mediated through the outer dynein arms (ODAs). Although the global redox state of the thioredox-inrelated ODA light chains LC3 and LC5 and the redox- sensitive Ca2+-binding subunit of the docking complex DC3 did not change upon light/dark transitions, we did observe significant alterations in their interactions with other flagellar components via mixed disulfides. These data indicate that redox poise directly affects ODAs and suggest that it may act in the control of flagellar motility. |
| DOI | 10.1083/jcb.200603019 |
| ISSN | 0021-9525 |
| NAID | 80019137812 |
| PMID | 16754958 |