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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1997 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | An isoform of the Golgi t-SNARE, syntaxin 5, with an endoplasmic reticulum retrieval signal |
| 執筆形態 | その他 |
| 掲載誌名 | Molecular Biology of the Cell |
| 出版社・発行元 | AMER SOC CELL BIOLOGY |
| 巻・号・頁 | 8(9),pp.1777-1787 |
| 著者・共著者 | Hui, N.,Nakamura, N.,Sönnichsen, B.,Shima, D.T.,Nilsson, T.,Warren, G. |
| 概要 | The early Golgi t-SNARE (target-membrane-associated soluble-N-ethylmaleimide-sensitive factor attachment protein receptor) syntaxin 5 is thought to specify the docking site for both COPI and COPII coated vesicles originating from the endoplasmic reticulum (ER) and COPI vesicles on the retrograde pathway. We now show that there are two forms of syntaxin 5 that appear to be generated from the same mRNA by alternative initiation of translation. The short form (35 kDa) corresponds to the published sequence. The long form (42 kDa) has an N-terminal cytoplasmic extension containing a predicted type II ER retrieval signal. When grafted onto a reporter molecule, this signal localized the construct to the ER. Biochemical fractionation and immunofluorescence microscopy showed that there was less of the long form in the Golgi apparatus and more in peripheral punctate structures, some of which colocalized with markers of the intermediate compartment. The predicted absence of the long form in budding yeast points to a function unique to higher organisms. |
| DOI | 10.1091/mbc.8.9.1777 |
| ISSN | 1059-1524 |
| Put Code(ORCID) | 19809425 |