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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Calcium-Dependent Flagellar Motility Activation in Chlamydomonas reinhardtii in Response to Mechanical Agitation |
| 執筆形態 | その他 |
| 掲載誌名 | CELL MOTILITY AND THE CYTOSKELETON |
| 出版社・発行元 | WILEY-LISS |
| 巻・号・頁 | 66(9),pp.736-742 |
| 担当区分 | 筆頭著者,責任著者 |
| 著者・共著者 | Ken-ichi Wakabayashi,Takahiro Ide,Ritsu Kamiya |
| 概要 | Flagellar beating in Chlamydomonas was found to be activated by mechanical stimulation. Immediately after a wild-type cell suspension was vortexed, the average swimming velocity of cells increased from 130 mu m/second to 150 mu m/second, due to an elevation of flagellar beat frequency from similar to 60 Hz to similar to 70 Hz without detectable change in the flagellar waveforms. This response required outer arm dynein. Treatment with EGTA, Ca(2+)-channel blockers, or mechanosensitive-channel blockers inhibited it. In demembranated and reactivated cell models, a modest increase in Ca(2+) concentration elevated the axonemal beat frequency. These data indicate that the mechanical agitation increases beat frequency because it causes Ca(2+) influx into flagella, which then activates outer arm dynein. Cell Motil. Cytoskeleton 66: 736-742, 2009. (C) 2009 Wiley-Liss, Inc. |
| DOI | 10.1002/cm.20402 |
| ISSN | 0886-1544 |
| PMID | 19544401 |