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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2014/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Multiple BiP Genes of Arabidopsis thaliana are Required for Male Gametogenesis and Pollen Competitiveness |
| 執筆形態 | その他 |
| 掲載誌名 | PLANT AND CELL PHYSIOLOGY |
| 出版社・発行元 | OXFORD UNIV PRESS |
| 巻・号・頁 | 55(4),pp.801-810 |
| 著者・共著者 | Daisuke Maruyama,Tomoyuki Sugiyama,Toshiya Endo,Shuh-ichi Nishikawa |
| 概要 | Immunoglobulin-binding protein (BiP) is a molecular chaperone of the heat shock protein 70 (Hsp70) family. BiP is localized in the endoplasmic reticulum (ER) and plays key roles in protein translocation, protein folding and quality control in the ER. The genomes of flowering plants contain multiple BiP genes. Arabidopsis thaliana has three BiP genes. BIP1 and BIP2 are ubiquitously expressed. BIP3 encodes a less well conserved BiP paralog, and it is expressed only under ER stress conditions in the majority of organs. Here, we report that all BiP genes are expressed and functional in pollen and pollen tubes. Although the bip1 bip2 double mutation does not affect pollen viability, the bip1 bip2 bip3 triple mutation is lethal in pollen. This result indicates that lethality of the bip1 bip2 double mutation is rescued by BiP3 expression. A decrease in the copy number of the ubiquitously expressed BiP genes correlates well with a decrease in pollen tube growth, which leads to reduced fitness of mutant pollen during fertilization. Because an increased protein secretion activity is expected to increase the protein folding demand in the ER, the multiple BiP genes probably cooperate with each other to ensure ER homeostasis in cells with active secretion such as rapidly growing pollen tubes. |
| DOI | 10.1093/pcp/pcu018 |
| ISSN | 0032-0781/1471-9053 |
| PMID | 24486762 |