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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1997 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The vesicle docking protein p115 binds GM130, a cis-Golgi matrix protein, in a mitotically regulated manner |
| 執筆形態 | その他 |
| 掲載誌名 | Cell |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 89(3),pp.445-455 |
| 著者・共著者 | Nakamura, N.,Lowe, M.,Levine, T.P.,Rabouille, C.,Warren, G. |
| 概要 | The docking of transport vesicles with their target membrane is thought to be mediated by p115. We show here that GM130, a cis-Golgi matrix protein, interacts specifically with p115 and so could provide a membrane docking site. Deletion analysis showed that the N-terminus binds to p115, whereas the C-terminus binds to Golgi membranes. Mitotic phosphorylation of GM130 or a peptide derived from the N-terminus prevented binding to p115. The peptide also inhibited the NSF- but not the p97-dependent reassembly of Golgi cisternae from mitotic fragments, unless it was mitotically phosphorylated. Together, these data provide a molecular explanation for the COPI-mediated fragmentation of the Golgi apparatus at the onset of mitosis. |
| DOI | 10.1016/S0092-8674(00)80225-1 |
| ISSN | 0092-8674 |
| Put Code(ORCID) | 19809426 |