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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Calcium oscillations in the olfactory nonsensory cells of the goldfish, Carassius auratus |
| 執筆形態 | その他 |
| 掲載誌名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 1790(12),pp.1681-1688 |
| 著者・共著者 | Takafumi Kawai,Hideki Abe,Ken-ichi Wakabayashi,Yoshitaka Oka |
| 概要 | Background: The olfactory nonsensory cells contribute to the maintenance of normal functions of the olfactory epithelium (OE). Specifically, the ciliated nonsensory cells of teleosts play important roles in the odorant detection by OE in aqueous environment. Their cilia show strong beating activities and cause water flow at the OE surface, making the detection of odorants by OE more efficient. Because intracellular Ca(2+) level has been reported to play an important role in ciliary beating, the ciliary beating activity may be regulated by intracellular Ca(2+) dynamics of these ciliated nonsensory cells.
Methods: We performed Ca(2+) imaging experiments to analyze the Ca(2+) dynamics in acutely dissociated OE cells of the goldfish. Furthermore, we examined the contribution of the Ca(2+) dynamics to the ciliary beating frequency (CBF) at the surface of the intact OE. Results: Olfactory nonsensory cells showed both spontaneous intracellular Ca(2+) oscillations and propagating intercellular Ca(2+) waves. Application of 2-aminoethoxydiphenylborate (2-APB), which antagonizes IP(3)-induced Ca(2+) release from intracellular stores suppressed these Ca(2+) oscillations. Furthermore, 2-APB application to the intact OE lamellae resulted in the decrease of CBF at the surface of the OE. Conclusions: These results indicate that spontaneous intracellular calcium oscillations persistently upregulate the ciliary beating at the surface of the OE in teleosts. General significance: Ciliary beating activity at the surface of OE can be regulated by the Ca(2+) dynamics of olfactory nonsensory cells. Because this ciliary movement causes inflow of external fluid into the nostril, this regulation is suggested to influence the efficiency of odorant detection by OE. (C) 2009 Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.bbagen.2009.09.009 |
| ISSN | 0304-4165 |