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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2002/08 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Stabilization of FtsH-unfolded protein complex by binding of ATP and blocking of protease |
| 執筆形態 | その他 |
| 掲載誌名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 296(1),pp.8-12 |
| 著者・共著者 | H Makyio,H Niwa,K Motohashi,H Taguchi,M Yoshida |
| 概要 | The function of an ATP-dependent membrane protease FtsH was investigated using the enzyme from Thermus thermophilus HB8. An FtsH mutant with replacement of Glu-419 in the zinc-binding motif by Cys lost the activity to digest casein, a model unfolded protein, and the small ATPase activity of this mutant was no longer stimulated by casein. In the presence of ATP or ATPgammaS, but not ADP, a mutant FtsH-unfolded protein complex was isolated, indicating that ATP binding, but not ATP hydrolysis, is required for FtsH to form a stable complex with an unfolded protein. The FtsH without mutation at Glu-419 did not produce a stable complex with casein in the presence of any nucleotides tested and therefore it appears that blocking proteolysis also contributes to stabilization of the complex. (C) 2002 Elsevier Science (USA). All rights reserved. |
| DOI | 10.1016/S0006-291X(02)00830-6 |
| ISSN | 0006-291X |
| NAID | 80015500561 |
| PMID | 12147219 |