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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2020/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Protocol for Disome Profiling to Survey Ribosome Collision in Humans and Zebrafish |
| 執筆形態 | その他 |
| 掲載誌名 | STAR Protocols |
| 掲載区分 | 国外 |
| 出版社・発行元 | Elsevier BV |
| 巻・号・頁 | 1(3),pp.100168-100168 |
| 著者・共著者 | Mari Mito,Yuichiro Mishima,Shintaro Iwasaki |
| 概要 | Ribosomes often encounter obstacles during translation elongation and thus collide with each other. Disome profiling, an optimized ribosome profiling method, specifically sequences the long ribosome footprints generated from collided ribosomes produced by the ribosome pause and thus allows the survey of sites in a genome-wide manner. This protocol details the procedure from lysate preparation of human tissue cultures and zebrafish embryos to sequencing library construction. For complete details on the use and execution of this protocol, please refer to Han et al. (2020). |
| DOI | 10.1016/j.xpro.2020.100168 |
| ISSN | 2666-1667 |
| PMID | 33377062 |