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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2007/02 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells |
| 執筆形態 | その他 |
| 掲載誌名 | MEDICAL MYCOLOGY |
| 出版社・発行元 | TAYLOR & FRANCIS LTD |
| 巻・号・頁 | 45(1),pp.73-79 |
| 著者・共著者 | Akiyoshi Sakai,Sumio Akifusa,Naoki Itano,Koji Kimata,Taro Kawamura,Takeyoshi Koseki,Tadamichi Takehara,Tatsuji Nishihara |
| 概要 | Candida albicans is both a commensal and a pathogen in the oral mucosa. Previous studies have indicated that epithelial cell-associated carbohydrate moiety can inhibit C albicans growth. In the present study, the mechanisms by which epithelial cells inhibit Candida growth were studied by examining the effect of hyaluronan (HA). A coculture of C albicans and KB cells or COS-7 cells inhibited in vitro growth of the fungus by 50-87% at an effector-to-target (E:T) ratio of 80:1. Removing extracellular HA by hyaluronidase caused a significant decrease in the anti-Candida activity of the cells. In addition anti-Candida activity was observed at 1 mu g/ml HA (2000 kDa). The antifungal activity of extracellular HA was further studied by transiently transfecting COS-7 cells with human HSA1, HSA2, or HSA3 in order to produce high levels of extracellular HA. All of the transfectants inhibited C albicans growth in vitro by 51-65% compared to 38% inhibition by the vector control (P < 0.05). These results suggest that the anti-Candida activity of epithelial-cells is mediated by extracellular HA. |
| DOI | 10.1080/13693780601039607 |
| ISSN | 1369-3786 |