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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/02 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2 |
| 執筆形態 | その他 |
| 掲載誌名 | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS |
| 出版社・発行元 | WILEY-BLACKWELL PUBLISHING, INC |
| 巻・号・頁 | 65(65:),pp.144-146 |
| 担当区分 | 責任著者 |
| 著者・共著者 | Takashi Kuzuhara,Daisuke Kise,Hiroko Yoshida,Takahiro Horita,Yoshimi Murazaki,Hiroko Utsunomiya,Hideaki Tsuge |
| 概要 | The C-terminal domain protein (amino-acid residues 535-759) of the PB2 subunit of the RNA-dependent RNA polymerase from the highly pathogenic influenza A virus was expressed as a soluble protein in Escherichia coli and crystallized using sodium formate as a precipitant. Data sets were collected from crystals of native and selenomethionine-substituted protein on the KEK NW12 beamline at the Photon Factory and the crystals diffracted to a maximum resolution of 2.44 angstrom for the SeMet-derivative crystal. The native crystals were found to belong to space group P3(2)21, with unit-cell parameters a = b = 52.5, c = 156.3 angstrom. The Matthews value (V(M)) was 2.7 angstrom(3) Da(-1), assuming the presence of one molecule in the asymmetric unit. The SeMet-derivative crystals were found to belong to the same space group, with unit-cell parameters a = b = 52.6, c = 156.4 angstrom. Attempts are being made to solve the structure by multi-wavelength anomalous dispersion phasing. |
| DOI | 10.1107/S1744309108042942 |
| ISSN | 1744-3091 |