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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2003/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Rotation of the proteolipid ring in the V-ATPase |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF BIOLOGICAL CHEMISTRY |
| 出版社・発行元 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 巻・号・頁 | 278(27),pp.24255-24258 |
| 著者・共著者 | K Yokoyama,M Nakano,H Imamura,M Yoshida,M Tamakoshi |
| 概要 | V0V1-ATPase is a proton-translocating ATPase responsible for acidification of eukaryotic intracellular compartments and for ATP synthesis in archaea and some eubacteria. We demonstrated recently the rotation of the central stalk subunits in V-1, a catalytic sector of V0V1-ATPase (Imamura, H., Nakano, M., Noji, H., Muneyuki, E., Ohkuma, S., Yoshida, M., and Yokoyama, K. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 2312-2315), but the rotation of the proteolipid ring, a predicted counterpart rotor in the membrane V-0 sector, has remained to be proven. V0V1-ATPase that retained sensitivity to N',N'-dicyclohexylcarbodiimide was isolated from Thermus thermophilus, immobilized onto a glass surface through the N termini of the A subunits of V-1, and decorated with a bead attached to a proteolipid subunit of V-0. Rotation of beads was observed in the presence of ATP, and direction of rotation was always counterclock-wise viewed from the membrane side. The rotation proceeded at similar to3.0 rev/s in average at 4 mM ATP and was abolished by N',N'-dicyclohexylcarbodiimide treatment. Thus, the rotation of the central stalk in V-1 accompanies rotation of a proteolipid ring of V-0 in the functioning V0V1-ATPase. |
| DOI | 10.1074/jbc.M303104200 |
| ISSN | 0021-9258 |
| PMID | 12707282 |