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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2007/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Versican, a major hyaluronan-binding component in the dermis, loses its hyaluronan-binding ability in solar elastosis |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF INVESTIGATIVE DERMATOLOGY |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 127(7),pp.1657-1663 |
| 著者・共著者 | Keiko Hasegawa,Masahiko Yoneda,Hiroko Kuwabara,Osamu Miyaishi,Naoki Itano,Akiko Ohno,Masahiro Zako,Zenzo Isogai |
| 概要 | Versican interacts with hyaluronan (HA) at its N-terminus and with fibrillin-1 at its C terminus. As versican in the dermis connects microfibrils to the HA-rich matrix for viscoelasticity, dermal diseases may involve destruction of these complexes. A recombinant versican protein, rVN, covering the HA binding region (HABR) of human versican and a polyclonal antibody, 6084, against rVN were prepared and characterized. Blotting analyses of skin extracts with 6084 and biotin-conjugated HA revealed that versican was a major HA-binding component in the dermis. Matrix metalloprotease-12, which is expressed in areas of solar elastosis, degraded versican and abrogated its HA-binding ability. Immunohistochemical analyses revealed that the elastic materials in solar elastosis lesions were negative for 6084, but positive for 21311, an antibody recognizing the C-terminus of versican, indicating loss of the HABR in the aggregated elastic fibers. This loss of the HA-binding ability of versican followed by HA exclusion may be responsible for the pathological and phenotypical changes observed in solar elastosis. |
| DOI | 10.1038/sj.jid.5700754 |
| ISSN | 0022-202X |