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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 発行・発表の年月 | 2025/05 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Msp1 and Pex19‐Pex3 cooperate to achieve correct localization of Pex15 to peroxisomes |
| 執筆形態 | 共著 |
| 掲載誌名 | The FEBS Journal |
| 掲載区分 | 国外 |
| 出版社・発行元 | Wiley |
| 巻・号・頁 | 292(16),4289-4313頁 |
| 担当区分 | 最終著者 |
| 国際共著 | 国際共著 |
| 著者・共著者 | Shunsuke Matsumoto,Yoshiki Kogure,Suzuka Ono,Tomoyuki Numata,Toshiya Endo |
| 概要 | Yeast Msp1 is a dual‐localized AAA‐ATPase on the mitochondrial outer membrane (OM) and peroxisomal membrane. We previously showed that Msp1 transfers mistargeted tail‐anchored (TA) proteins from mitochondria to the endoplasmic reticulum (ER) for degradation or delivery to their original destinations. However, the mechanism by which Msp1 in mitochondria and peroxisomes handles authentic peroxisomal TA proteins remains unclear. We show that newly synthesized Pex15 is targeted to peroxisomes primarily via the Pex19‐ and Pex3‐dependent pathway. Mistargeted Pex15 on the mitochondrial OM is extracted by mitochondrial Msp1 and transferred to the ER via the guided‐entry of TA proteins pathway for degradation or to peroxisomes via the Pex19‐Pex3 pathway. Intriguingly, endogenous Pex15 localized in peroxisomes is also extracted from the membranes by peroxisomal Msp1 but returns to peroxisomes via the Pex19‐Pex3 pathway. These results suggest that correct Pex15 localization to peroxisomes relies on not only the initial targeting by Pex19‐Pex3 but also the constant re‐routing by Msp1 and Pex19‐Pex3. |
| DOI | 10.1111/febs.70132 |
| ISSN | 1742-464X/1742-4658 |
| PermalinkURL | https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/febs.70132 |