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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2024 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Analysis of protein trafficking between mitochondria and the endoplasmic reticulum by fluorescence microscopy. |
| 執筆形態 | 共著 |
| 掲載誌名 | Methods in enzymology |
| 掲載区分 | 国外 |
| 巻・号・頁 | 707,pp.153-171 |
| 担当区分 | 責任著者 |
| 著者・共著者 | Shunsuke Matsumoto, Suzuka Ono, Toshiya Endo |
| 概要 | Precise protein localization is essential for normal cellular functions. However, recent studies have revealed that protein targeting is error-prone, and tail-anchored proteins mistargeted to mitochondria are transferred to the endoplasmic reticulum (ER) by an ATPase Msp1 (yeast)/ATAD1 (human) in the mitochondrial outer membrane for further quality examination in the ER to determine their fate, degradation or re-targeting. Analysis of the inter-organelle transfer of proteins requires a combination of time-lapse fluorescence microscopy and a system to achieve regulation of the protein levels of both transfer substrates and factors regulating the transfer in a coordinated manner at precise timing. This can be achieved by using a promoter switch for expression and acute depletion of involved factors through the degron-based proteasome system. In this chapter, we will describe methods to analyze inter-organelle protein transfer by fluorescence microscope within living yeast cells, by using the example of Msp1-mediated transfer of mistargeted proteins from mitochondria to the ER. |
| DOI | 10.1016/bs.mie.2024.07.041 |
| PMID | 39488373 |