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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2024/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Chemical inhibition of phosphatidylcholine biogenesis reveals its role in mitochondrial division. |
| 執筆形態 | その他 |
| 掲載誌名 | iScience |
| 掲載区分 | 国外 |
| 巻・号・頁 | 27(3),pp.109189-109189 |
| 著者・共著者 | Hiroya Shiino,Shinya Tashiro,Michiko Hashimoto,Yuki Sakata,Takamitsu Hosoya,Toshiya Endo,Hirotatsu Kojima,Yasushi Tamura |
| 概要 | Phospholipids are major components of biological membranes and play structural and regulatory roles in various biological processes. To determine the biological significance of phospholipids, the use of chemical inhibitors of phospholipid metabolism offers an effective approach; however, the availability of such compounds is limited. In this study, we performed a chemical-genetic screening using yeast and identified small molecules capable of inhibiting phosphatidylcholine (PC) biogenesis, which we designated PC inhibitors 1, 2, 3, and 4 (PCiB-1, 2, 3, and 4). Biochemical analyses indicated that PCiB-2, 3, and 4 inhibited the phosphatidylethanolamine (PE) methyltransferase activity of Cho2, whereas PCiB-1 may inhibit PE transport from mitochondria to the endoplasmic reticulum (ER). Interestingly, we found that PCiB treatment resulted in mitochondrial fragmentation, which was suppressed by expression of a dominant-negative mutant of the mitochondrial division factor Dnm1. These results provide evidence that normal PC biogenesis is important for the regulation of mitochondrial division. |
| DOI | 10.1016/j.isci.2024.109189 |
| PMID | 38420588 |