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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2015/08 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Structural and mechanistic insights into phospholipid transfer by Ups1-Mdm35 in mitochondria |
| 執筆形態 | その他 |
| 掲載誌名 | NATURE COMMUNICATIONS |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 6,pp.7922 |
| 著者・共著者 | Yasunori Watanabe,Yasushi Tamura,Shin Kawano,Toshiya Endo |
| 概要 | Eukaryotic cells are compartmentalized into membrane-bounded organelles whose functions rely on lipid trafficking to achieve membrane-specific compositions of lipids. Here we focused on the Ups1-Mdm35 system, which mediates phosphatidic acid (PA) transfer between the outer and inner mitochondrial membranes, and determined the X-ray structures of Mdm35 and Ups1-Mdm35 with and without PA. The Ups1-Mdm35 complex constitutes a single domain that has a deep pocket and flexible O-loop lid. Structure-based mutational analyses revealed that a basic residue at the pocket bottom and the O-loop lid are important for PA extraction from the membrane following Ups1 binding. Ups1 binding to the membrane is enhanced by the dissociation of Mdm35. We also show that basic residues around the pocket entrance are important for Ups1 binding to the membrane and PA extraction. These results provide a structural basis for understanding the mechanism of PA transfer between mitochondrial membranes. |
| DOI | 10.1038/ncomms8922 |
| ISSN | 2041-1723 |
| PMID | 26235513 |