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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2015 |
| 形態種別 | 論文集（書籍）内論文 |
| 査読 | 査読あり |
| 標題 | Axonemal motility in Chlamydomonas |
| 執筆形態 | その他 |
| 掲載誌名 | METHODS IN CILIA & FLAGELLA |
| 出版社・発行元 | ELSEVIER ACADEMIC PRESS INC |
| 巻・号・頁 | 127,pp.387-402 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | Ken-ichi Wakabayashi,Ritsu Kamiya |
| 概要 | Motile cilia and flagella rapidly propagate bending waves and produce water flow over the cell surface. Their function is important for the physiology and development of various organisms including humans. The movement is based on the sliding between outer doublet microtubules driven by axonemal dyneins, and is regulated by various axonemal components and environmental factors. For studies aiming to elucidate the mechanism of cilia/flagella movement and regulation, Chlamydomonas is an invaluable model organism that offers a variety of mutants. This chapter introduces standard methods for studying Chlamydomonas flagellar motility including analysis of swimming paths, measurements of swimming speed and beat frequency, motility reactivation in demembranated cells (cell models), and observation of microtubule sliding in disintegrating axonemes. Most methods may be easily applied to other organisms with slight modifications of the medium conditions. |
| DOI | 10.1016/bs.mcb.2014.12.002 |
| ISSN | 0091-679X |
| PMID | 25837401 |