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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/10 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Structural basis for the Helicobacter pylori-carcinogenic TNF-alpha-inducing protein |
| 執筆形態 | その他 |
| 掲載誌名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 388(2),pp.193-198 |
| 担当区分 | 筆頭著者,責任著者 |
| 著者・共著者 | Hideaki Tsuge,Toshiharu Tsurumura,Hiroko Utsunomiya,Daisuke Kise,Takashi Kuzuhara,Tatsuro Watanabe,Hirota Fujiki,Masami Suganuma |
| 概要 | Stomach cancer is strongly associated with infection by Helicobacter pylori. In 2005, we identified a new H. pylori gene encoding a TNF-alpha inducing protein (Tip alpha) that acts as a carcinogenic factor. Tip alpha is secreted from H. pylori as a homodimer whose subunits are linked by disulfide bonds. We also characterized a Tip alpha deletion mutant (del-Tip alpha) that lacks the N-terminal six amino acid residues (LQACTC), including two cysteines (C5 and C7) that form disulfide bonds, but nonetheless shows a weak ability to induce TNF-alpha expression. Here we report that del-Tip alpha has a novel elongated structure containing a 40-angstrom-long alpha helix, and forms a heart-shaped homodimer via non-covalent bonds. Moreover, their circular dichroism spectra strongly suggest that the structures of the del-Tip alpha and Tip alpha homodimers are very similar. del-Tip alpha's unique mode of dimer formation provides important insight into protein-protein interactions and into the mechanism underlying the carcinogenicity of H. pylori infection. (C) 2009 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.bbrc.2009.07.121 |
| ISSN | 0006-291X/1090-2104 |