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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Identification of the agg1 mutation responsible for negative phototaxis in a “wild-type” strain of Chlamydomonas reinhardtii |
| 執筆形態 | その他 |
| 掲載誌名 | Biochemistry and Biophysics Reports |
| 出版社・発行元 | Elsevier B.V. |
| 巻・号・頁 | 7,pp.379-385 |
| 担当区分 | 責任著者 |
| 著者・共著者 | Takahiro Ide,Shota Mochiji,Noriko Ueki,Katsushi Yamaguchi,Shuji Shigenobu,Masafumi Hirono,Ken-ichi Wakabayashi |
| 概要 | The unicellular green alga Chlamydomonas reinhardtii is a model organism for various studies in biology. CC-124 is a laboratory strain widely used as a wild type. However, this strain is known to carry agg1 mutation, which causes cells to swim away from the light source (negative phototaxis), in contrast to the cells of other wild-type strains, which swim toward the light source (positive phototaxis). Here we identified the causative gene of agg1 (AGG1) using AFLP-based gene mapping and whole genome next-generation sequencing. This gene encodes a 36-kDa protein containing a Fibronectin type III domain and a CHORD-Sgt1 (CS) domain. The gene product is localized to the cell body and not to flagella or basal body. |
| DOI | 10.1016/j.bbrep.2016.07.016 |
| ISSN | 2405-5808/2405-5808 |