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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2016/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | A phospholipid transfer function of ER-mitochondria encounter structure revealed in vitro |
| 執筆形態 | その他 |
| 掲載誌名 | SCIENTIFIC REPORTS |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 6,pp.30777 |
| 著者・共著者 | Rieko Kojima,Toshiya Endo,Yasushi Tamura |
| 概要 | As phospholipids are synthesized mainly in the endoplasmic reticulum ( ER) and mitochondrial inner membranes, how cells properly distribute specific phospholipids to diverse cellular membranes is a crucial problem for maintenance of organelle-specific phospholipid compositions. Although the ER-mitochondria encounter structure (ERMES) was proposed to facilitate phospholipid transfer between the ER and mitochondria, such a role of ERMES is still controversial and awaits experimental demonstration. Here we developed a novel in vitro assay system with isolated yeast membrane fractions to monitor phospholipid exchange between the ER and mitochondria. With this system, we found that phospholipid transport between the ER and mitochondria relies on membrane intactness, but not energy sources such as ATP, GTP or the membrane potential across the mitochondrial inner membrane. We further found that lack of the ERMES component impairs the phosphatidylserine transport from the ER to mitochondria, but not the phosphatidylethanolamine transport from mitochondria to the ER. This in vitro assay system thus offers a powerful tool to analyze the non-vesicular phospholipid transport between the ER and mitochondria. |
| DOI | 10.1038/srep30777 |
| ISSN | 2045-2322 |
| PMID | 27469264 |