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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2005 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Convergence of cell cycle regulation and growth factor signals on GRASP65 |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Biological Chemistry |
| 出版社・発行元 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 巻・号・頁 | 280(24),pp.23048-23056 |
| 著者・共著者 | Yoshimura, S.-I.,Yoshioka, K.,Barr, F.A.,Lowe, M.,Nakayama, K.,Ohkuma, S.,Nakamura, N. |
| 概要 | Together with other Golgi matrix components, GRASP65 contributes to the stacking of Golgi cisternae in interphase cells. During mitosis, GRASP65 is heavily phosphorylated, and in turn, cisternal stacking is inhibited leading to the breakdown of the Golgi apparatus. Here we show that GRASP65 is phosphorylated on serine 277 in interphase cells, and this is strongly enhanced in response to the addition of serum or epidermal growth factor. This is directly mediated by ERK suggesting that GRASP65 has some role in growth factor signal transduction. Phosphorylation of Ser-277 is also dramatically increased during mitosis, however this is mediated by Cdk1 and not by ERK. The microinjection of recombinant GRASP65 without N-terminal myristoylation or a peptide fragment containing Ser-277 into the cytosol of normal rat kidney cells inhibits passage through mitosis. This effect is abolished when Ser-277 is replaced with alanine suggesting the phosphorylation of Ser-277 plays an important role in cell cycle regulation. The convergence of cell cycle regulation and growth factor signals on GRASP65 Ser-277 suggests that GRASP65 may function as a signal integrator controlling the cell growth. |
| DOI | 10.1074/jbc.M502442200 |
| ISSN | 0021-9258 |
| Put Code(ORCID) | 19809411 |