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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2005 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Depletion of vesicle-tethering factor p115 causes mini-stacked Golgi fragments with delayed protein transport |
| 執筆形態 | その他 |
| 掲載誌名 | Biochemical and Biophysical Research Communications |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 338(2),pp.1268-1274 |
| 著者・共著者 | Sohda, M.,Misumi, Y.,Yoshimura, S.-I.,Nakamura, N.,Fusano, T.,Sakisaka, S.,Ogata, S.,Fujimoto, J.,Kiyokawa, N.,Ikehara, Y. |
| 概要 | Depletion of p115 with small interfering RNA caused fragmentation of the Golgi apparatus, resulting in dispersed distribution of stacked short cisternae and a vesicular structure (mini-stacked Golgi). The mini-stacked Golgi with cis- and trans-organization is functional in protein transport and glycosylation, although secretion is considerably retarded in p] 15 knockdown cells. The fragmented Golgi was further disrupted by treatment with breferdin A and reassembled into the mini-stacked Golgi by removal of the drug, as observed in control cells. In addition, p115 knockdown cells maintained retrograde transport from the Golgi to the endoplasmic reticulum, although the rate was not as efficient as in control cells. While no alternation of microtubule networks was found in p115 knockdown cells, the fragmented Golgi resembled those in cells treated with anti-microtubule drugs. The results suggest that p115 is involved in vesicular transport between endoplasmic reticulum and the Golgi, along with microtubule networks. (c) 2005 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.bbrc.2005.10.084 |
| ISSN | 0006-291X |
| Put Code(ORCID) | 19809410 |