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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2005/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus |
| 執筆形態 | その他 |
| 掲載誌名 | EMBO JOURNAL |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 24(22),pp.3974-3983 |
| 著者・共著者 | H Makyio,R Iino,C Ikeda,H Imamura,M Tamakoshi,M Iwata,D Stock,RA Bernal,EP Carpenter,M Yoshida,K Yokoyama,S Iwata |
| 概要 | The crystal structure of subunit F of vacuole-type ATPase/synthase (prokaryotic V-ATPase) was determined to of 2.2 angstrom resolution. The subunit reveals unexpected structural similarity to the response regulator proteins that include the Escherichia coli chemotaxis response regulator CheY. The structure was successfully placed into the low-resolution EM structure of the prokaryotic holo-V-ATPase at a location indicated by the results of cross-linking experiments. The crystal structure, together with the single-molecule analysis using fluorescence resonance energy transfer, showed that the subunit F exhibits two conformations, a 'retracted' form in the absence and an 'extended' form in the presence of ATP. Our results postulated that the subunit F is a regulatory subunit in the V-ATPase. |
| DOI | 10.1038/sj.emboj.7600859 |
| ISSN | 0261-4189 |
| PMID | 16281059 |