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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Detergent-extracted Volvox model exhibits an anterior–posterior gradient in flagellar Ca2+ sensitivity |
| 執筆形態 | その他 |
| 掲載誌名 | Proceedings of the National Academy of Sciences of the United States of America |
| 出版社・発行元 | National Academy of Sciences |
| 巻・号・頁 | 115(5),pp.E1061-E1068 |
| 担当区分 | 責任著者 |
| 著者・共著者 | Noriko Ueki,Ken-ichi Wakabayashi |
| 概要 | Volvox rousseletii is a multicellular spheroidal green alga containing ˜5,000 cells, each equipped with two flagella (cilia). This organism shows striking photobehavior without any known intercellular communication. To help understand how the behavior of flagella is regulated, we developed a method to extract the whole organism with detergent and reactivate its flagellar motility. Upon addition of ATP, demembranated flagella (axonemes) in the spheroids actively beat and the spheroids swam as if they were alive. Under Ca2+-free conditions, the axonemes assumed planar and asymmetrical waveforms and beat toward the posterior pole, as do live spheroids in the absence of light stimulation. In the presence of 10−6 M Ca2+, however, most axonemes beat three-dimensionally toward the anterior pole, similar to flagella in photostimulated live spheroids. This Ca2+-dependent change in flagellar beating direction was more conspicuous near the anterior pole of the spheroid, but was not observed near the posterior pole. This anterior–posterior gradient of flagellar Ca2+ sensitivity may explain the mechanism of V. rousseletii photobehavior. |
| DOI | 10.1073/pnas.1715489115 |
| ISSN | 1091-6490 |
| PMID | 29311312 |