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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2005/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Rotation scheme of V-1-motor is different from that of F-1-motor |
| 執筆形態 | その他 |
| 掲載誌名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
| 出版社・発行元 | NATL ACAD SCIENCES |
| 巻・号・頁 | 102(50),pp.17929-17933 |
| 著者・共著者 | H Imamura,M Takeda,S Funamoto,K Shimabukuro,M Yoshida,K Yokoyama |
| 概要 | V-1, a water-soluble portion of vacuole-type ATPase (V-ATPase), is an ATP-driven rotary motor, similar to F-1-ATPase. Hydrolysis of ATP is coupled to unidirectional rotation of the central rotor D and F subunits relative to the A(3)B(3) cylinder. In this study, we analyzed the rotation kinetics of V-1 in detail. At low ATP concentrations, the D subunit rotated stepwise, pausing every 120 degrees. The dwell time between steps revealed that V-1 consumes one ATP per 120 degrees step. V-1 generated torque of approximate to 35 pN nm, slightly lower than the approximate to 46 pN nm measured for F-1. Noticeably, the angles for both ATP cleavage and binding were apparently the same in V-1, in sharp contrast to F-1, which cleaves ATP at 80 degrees posterior to the binding of ATP. Thus, the mechanochemical cycle of V-1 has marked differences to that of F-1. |
| DOI | 10.1073/pnas.0507764102 |
| ISSN | 0027-8424 |
| PMID | 16330761 |