|
ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2006/08 |
| 形態種別 | 研究論文 |
| 標題 | Alteration of developmental program in Paramecium by treatment with trichostatin A: A possible involvement of histone modification |
| 執筆形態 | その他 |
| 掲載誌名 | PROTIST |
| 出版社・発行元 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 巻・号・頁 | 157(3),pp.303-314 |
| 著者・共著者 | Naomi Kimura,Kazuyuki Mikami,Nobuhiro Nakamura,Hiroshi Endoh |
| 概要 | In the ciliate Paramecium caudatum, the somatic macronucleus for the next generation differentiates from a zygotic germinal micronucleus during conjugation. Subsequently, progeny are programmed to enter a sexually "immature" period, during which cells do not mate. This programming occurs at a critical period during the fifth cell cycle after conjugation. Here, we show that treatment with trichostatin A, a histone deacetylase inhibitor, leads to a partial recovery of mating reactivity in immature cells, suggesting that histone acetylation can affect the developmental program. In addition, immunostaining demonstrated that Lys 4 methylation of histone H3 was absent from or present at an undetectable level in the nascent macronucleus during the first cell cycle after conjugation. The methylation level gradually increased in proportion to de novo DNA synthesis, until the new macronucleus reached the maximum level of methylation, concomitant with its full maturation. A link between gene expression and Lys 4 methylation is indirectly supported by the observation that a transcriptionally active gene was enriched by chromatin immunoprecipitation with an antibody directed against methyl-Lys 4 H3, whereas a silent gene was not. These results provide evidence that histone modification plays a key role in the regulation of gene expression and the developmental programming that determine sexual immaturity after conjugation. (c) 2006 Elsevier GmbH. All rights reserved. |
| DOI | 10.1016/j.protis.2006.05.008 |
| ISSN | 1434-4610 |