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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Identification of thioredoxin targeted proteins using thioredoxin single cysteine mutant-immobilized resin |
| 執筆形態 | その他 |
| 掲載誌名 | Methods in Molecular Biology |
| 出版社・発行元 | Humana Press |
| 巻・号・頁 | 479(117-131),pp.117-131 |
| 著者・共著者 | Ken Motohashi,Patrick G.N. Romano,Toru Hisabori |
| 概要 | Thioredoxins (Trx) are a ubiquitous family of proteins that modulate the enzymatic activity of their substrate proteins by redox regulation. This is achieved by reduction of a disulfide bond within their target proteins. A conserved pair of cysteine residues in Trx is required for catalysis of the dithiol-disulfide exchange with their target proteins. A single-cysteine mutant capable of forming a stable mixed disulfide bond with target proteins was immobilized on resin and used to capture potential target proteins. By using this method, a number of previously unidentified Trx-target protein candidates were captured from various organisms and organelles. Following the development of this technique, more than one hundred proteins have been reported as potential Trx targets, allowing significant progress to be made in our knowledge and understanding of Trx-target proteins. © 2009 Humana Press. |
| DOI | 10.1007/978-1-59745-289-2_8 |
| ISSN | 1064-3745 |
| PMID | 19083171 |