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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2018/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Thioredoxin regulates G6PDH activity by changing redox states of OpcA in the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 |
| 執筆形態 | その他 |
| 掲載誌名 | Biochemical Journal |
| 出版社・発行元 | Portland Press Ltd |
| 巻・号・頁 | 475(6),pp.1091-1105 |
| 著者・共著者 | Shoko Mihara,Hitomi Wakao,Keisuke Yoshida,Akiyoshi Higo,Kazunori Sugiura,Akihiro Tsuchiya,Jiro Nomata,Ken-Ichi Wakabayashi,Toru Hisabori |
| 概要 | Glucose 6-phosphate dehydrogenase (G6PDH) catalyzes the first reaction in the oxidative pentose phosphate pathway. In green plant chloroplasts, G6PDH is a unique redox-regulated enzyme, since it is inactivated under the reducing conditions. This regulation is accomplished using a redox-active cysteine pair, which is conserved in plant G6PDH. The inactivation of this enzyme under conditions of light must be beneficial to prevent release of CO2 from the photosynthetic carbon fixation cycle. In the filamentous, heterocyst-forming, nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120 (Anabaena 7120), G6PDH plays a pivotal role in providing reducing power for nitrogenase, and its activity is also reported to be suppressed by reduction, though Anabaena G6PDH does not conserve the critical cysteines for regulation. Based on the thorough analyses of the redox regulation mechanisms of G6PDH from Anabaena 7120 and its activator protein OpcA, we found that m-type thioredoxin regulates G6PDH activity by changing the redox states of OpcA. Mass spectrometric analysis and mutagenesis studies indicate that Cys393 and Cys399 of OpcA are responsible for the redox regulation property of this protein. Moreover, in vivo analyses of the redox states of OpcA showed that more than half of the OpcA is present as an oxidized form, even under conditions of light, when cells are cultured under the nitrogen-fixing conditions. This redox regulation of OpcA might be necessary to provide reducing power for nitrogenase by G6PDH in heterocysts even during the day. |
| DOI | 10.1042/BCJ20170869 |
| ISSN | 1470-8728 |
| PMID | 29440317 |