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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2011/11 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Structure and stability of Gyuba, a beta-lactoglobulin chimera |
| 執筆形態 | その他 |
| 掲載誌名 | PROTEIN SCIENCE |
| 出版社・発行元 | WILEY-BLACKWELL |
| 巻・号・頁 | 20(11),pp.1867-1875 |
| 著者・共著者 | Hideaki Ohtomo,Tsuyoshi Konuma,Hiroko Utsunoiya,Hideaki Tsuge,Masamichi Ikeguchi |
| 概要 | beta-lactoglobulin (LG) contains nine beta-strands (strands A-I) and one a-helix. Strands A-H form a beta-barrel. At neutral pH, equine LG (ELG) is monomeric, whereas bovine LG (BLG) is dimeric, and the I-strands of its two subunits form an intermolecular beta-sheet. We previously constructed a chimeric ELG in which the sequence of the I-strand was replaced with that of BLG. This chimera did not dimerize. For this study, we constructed the new chimera we call Gyuba (which means cow and horse in Japanese). The amino acid sequence of Gyuba includes the sequences of the BLG secondary structures and those of the ELG loops. The crystal structure of Gyuba is very similar to that of BLG and indicates that Gyuba dimerizes via the intermolecular beta-sheet formed by the two I-strands. Thus, the entire arrangement of the secondary structural elements is important for LG dimer formation. |
| DOI | 10.1002/pro.720 |
| ISSN | 0961-8368 |