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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2007 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Autophagosome-lysosome fusion depends on the pH in acidic compartments in CHO cells |
| 執筆形態 | その他 |
| 掲載誌名 | Autophagy |
| 出版社・発行元 | TAYLOR & FRANCIS INC |
| 巻・号・頁 | 3(2),pp.154-157 |
| 著者・共著者 | Kawai, A.,Uchiyama, H.,Takano, S.,Nakamura, N.,Ohkuma, S. |
| 概要 | Autophagy is the bulk degradation of cytoplasmic constituents in response to starvation and other environmental or intracellular cues. During this process, most of the cytoplasm is sequestered into autophagosomes, which then fuse with lysosomes where the degradation of the sequestered material proceeds. We investigated the relationship between autophagosome-lysosome fusion and the pH in acidic compartments by visualizing the fusion process using fluorescence in CHO cells. In this experiment, mitochondria were labeled with GFP by transfecting CHO cells with the presequence of ornithine trans-carbomylase, and lysosomes were labeled with Texas Red Dextran; any fusion was identified by the colocalization of mitochondria (in autophagosomes) and lysosomes using fluorescence microscopy. When CHO cells were treated with rapamycin or starvation medium to induce autophogy, the colocalization of fluorescence was observed. Whereas when they were treated with 3-MA, an inhibitor of autophagy, the colocalization disappeared. We conclude that the colocalization reflects the fusion of autophagosomes and lysosomes. Moreover, when the CHO cells were treated with drugs that increase the pH of acidic compartments, the colocalization disappeared. This suggests that the autophagosome-lysosome fusion is inhibited by increasing pH in acidic compartments independently of V-ATPase activity in CHO cells. |
| DOI | 10.4161/auto.3634 |
| ISSN | 1554-8627/1554-8635 |
| Put Code(ORCID) | 19809403 |