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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2007 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | The interaction of two tethering factors, p115 and COG complex, is required for golgi integrity |
| 執筆形態 | その他 |
| 掲載誌名 | Traffic |
| 出版社・発行元 | BLACKWELL PUBLISHING |
| 巻・号・頁 | 8(3),pp.270-284 |
| 著者・共著者 | Sohda, M.,Misumi, Y.,Yoshimura, S.-I.,Nakamura, N.,Fusano, T.,Ogata, S.,Sakisaka, S.,Ikehara, Y. |
| 概要 | The vesicle-tethering protein p115 functions in endoplasmic reticulum-Golgi trafficking. We explored the function of homologous region 2 (HR2) of the p115 head domain that is highly homologous with the yeast counterpart, Uso1p. By expression of p115 mutants in p115 knockdown (KD) cells, we found that deletion of HR2 caused an irregular assembly of the Golgi, which consisted of a cluster of mini-stacked Golgi fragments, and gathered around microtubule-organizing center in a microtubule-dependent manner. Protein interaction analyses revealed that p115 HR2 interacted with Cog2, a subunit of the conserved oligomeric Golgi (COG) complex that is known another putative cis-Golgi vesicle-tethering factor. The interaction between p115 and Cog2 was found to be essential for Golgi ribbon reformation after the disruption of the ribbon by p115 KD or brefeldin A treatment and recovery by re-expression of p115 or drug wash out, respectively. The interaction occurred only in interphase cells and not in mitotic cells. These results strongly suggested that p115 plays an important role in the biogenesis and maintenance of the Golgi by interacting with the COG complex on the cis-Golgi in vesicular trafficking. |
| DOI | 10.1111/j.1600-0854.2006.00530.x |
| ISSN | 1398-9219 |
| Put Code(ORCID) | 19809404 |