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モトハシ タケシ
MOTOHASHI TAKESHI
本橋 健 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2013/06 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Systematic exploration of thioredoxin target proteins in plant mitochondria |
| 執筆形態 | その他 |
| 掲載誌名 | Plant and Cell Physiology |
| 巻・号・頁 | 54(6),pp.875-892 |
| 著者・共著者 | Keisuke Yoshida,Ko Noguchi,Ken Motohashi,Toru Hisabori |
| 概要 | The thioredoxin (Trx) system is known to play a pivotal role in cellular redox regulation, but its target proteins in plant mitochondria remain largely uncharacterized. In this study, we systemically screened Trx target candidates in plant mitochondria. Mitochondrial protein extracts were prepared from Arabidopsis shoots, spinach leaves and potato tubers, and then subfractionated into soluble matrix and insoluble membrane fractions. Protein extracts were loaded onto an affinity column immobilizing Arabidopsis mitochondria-localized o-type Trx mutant protein, in which one of two internal cysteines at the active site was substituted by serine. Proteins forming mixed-disulfide intermediates with the mutated Trx were identified by proteomic approaches. This procedure allowed the determination of 101 Trx target candidate proteins involved in a broad spectrum of mitochondrial processes. Furthermore, biochemical assay revealed that one of the potential Trx target proteins, alternative oxidase, is actually redox regulated by Trx. This study provides insights into the regulatory mechanism of diverse functions in mitochondrial biology that are mediated through the Trx system. © 2013 The Author. |
| DOI | 10.1093/pcp/pct037 |
| ISSN | 0032-0781 |
| PMID | 23444301 |
| Put Code(ORCID) | 21490255 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84878846165&partnerID=MN8TOARS |
| researchmap用URL | http://orcid.org/0000-0002-8414-2836 |