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若林 憲一 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2019/03 |
| 形態種別 | その他 |
| 査読 | 査読あり |
| 標題 | Inner lumen proteins stabilize doublet microtubules in cilia and flagella. |
| 執筆形態 | その他 |
| 掲載誌名 | Nature communications |
| 掲載区分 | 国外 |
| 巻・号・頁 | 10(1),pp.1143-1143 |
| 著者・共著者 | Mikito Owa,Takayuki Uchihashi,Haru-Aki Yanagisawa,Takashi Yamano,Hiro Iguchi,Hideya Fukuzawa,Ken-Ichi Wakabayashi,Toshio Ando,Masahide Kikkawa |
| 概要 | Motile cilia are microtubule-based organelles that play important roles in most eukaryotes. Although axonemal microtubules are sufficiently stable to withstand their beating motion, it remains unknown how they are stabilized while serving as tracks for axonemal dyneins. To address this question, we have identified two uncharacterized proteins, FAP45 and FAP52, as microtubule inner proteins (MIPs) in Chlamydomonas. These proteins are conserved among eukaryotes with motile cilia. Using cryo-electron tomography (cryo-ET) and high-speed atomic force microscopy (HS-AFM), we show that lack of these proteins leads to a loss of inner protrusions in B-tubules and less stable microtubules. These protrusions are located near the inner junctions of doublet microtubules and lack of both FAP52 and a known inner junction protein FAP20 results in detachment of the B-tubule from the A-tubule, as well as flagellar shortening. These results demonstrate that FAP45 and FAP52 bind to the inside of microtubules and stabilize ciliary axonemes. |
| DOI | 10.1038/s41467-019-09051-x |
| PMID | 30850601 |