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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Crystal structures of dye-decolorizing peroxidase with ascorbic acid and 2,6-dimethoxyphenol |
| 執筆形態 | その他 |
| 掲載誌名 | FEBS Letters |
| 出版社・発行元 | ELSEVIER SCIENCE BV |
| 巻・号・頁 | 586(24),pp.4351-4356 |
| 著者・共著者 | Yoshida, T.,Tsuge, H.,Hisabori, T.,Sugano, Y. |
| 概要 | The structure of dye-decolorizing peroxidase (DyP)-type peroxidase differs from that of other peroxidase families, indicating that DyP-type peroxidases have a different reaction mechanism. We have determined the crystal structures of DyP with ascorbic acid and 2,6-dimethoxyphenol at 1.5 and 1.4 angstrom, respectively. The common binding site for both substrates was located at the entrance of the second cavity leading from the DyP molecular surface to heme. This resulted in a hydrogen bond network connection between each substrate and the heme distal side. This network consisted of water molecules occupying the second cavity, heme 6-propionate, Arg329, and Asn313. This network is consistent with the proton transfer pathway from substrate to DyP. (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
| DOI | 10.1016/j.febslet.2012.10.049 |
| ISSN | 0014-5793 |
| Put Code(ORCID) | 6110937 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84870571391&partnerID=MN8TOARS |