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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2010/09 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Exercise Effects on Methylation of ASC Gene |
| 執筆形態 | その他 |
| 掲載誌名 | INTERNATIONAL JOURNAL OF SPORTS MEDICINE |
| 出版社・発行元 | GEORG THIEME VERLAG KG |
| 巻・号・頁 | 31(9),pp.671-675 |
| 著者・共著者 | K. Nakajima,M. Takeoka,M. Mori,S. Hashimoto,A. Sakurai,H. Nose,K. Higuchi,N. Itano,M. Shiohara,T. Oh,S. Taniguchi |
| 概要 | Chronic moderate exercise has been reported to reduce pro-inflammatory cytokines. To analyze the molecular mechanisms by which training exerts these effects, the epigenetic influences of age and exercise on the ASC gene, which is responsible for IL-1 beta and IL-18 secretion, were investigated by ASC gene methylation. Further, the relationship between carcinogenesis and exercise, and methylation of the p15 tumor suppressive gene was also analyzed. High-intensity interval walking exercise, consisting of 3 min low-intensity walking at 40% of peak aerobic capacity followed by a 3 min high-intensity walking period above 70% of peak aerobic capacity, was continued for 6 months. Peripheral blood DNA extracts from young control (n = 34), older control (n = 153), and older exercise (n = 230) groups were then analyzed by pyrosequencing for DNA methylation. Methylation of ASC decreased significantly with age (young control vs. older control, p < 0.01), which is indicative of an age-dependent increase in ASC expression. Compared to the older control group, the degree of ASC methylation was higher in the older exercise group (older control vs. older exercise: p < 0.01), and presumably lower ASC expression. Neither exercise nor age affected the methylation of the p15. In summary, chronic moderate exercise appears to attenuate the age-dependent decrease in ASC methylation, implying suppression of excess pro-inflammatory cytokines through reduction of ASC expression. |
| DOI | 10.1055/s-0029-1246140 |
| ISSN | 0172-4622 |