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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2007/12 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus |
| 執筆形態 | その他 |
| 掲載誌名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
| 出版社・発行元 | NATL ACAD SCIENCES |
| 巻・号・頁 | 104(51),pp.20256-20261 |
| 著者・共著者 | Masashi Toei,Christoph Gerle,Masahiro Nakano,Kazutoshi Tani,Nobuhiko Gyobu,Masatada Tamakoshi,Nobuhito Sone,Masasuke Yoshida,Yoshinori Fujiyoshi,Kaoru Mitsuoka,Ken Yokoyama |
| 概要 | ATP synthesis by V-ATPase from the thermophilic bacterium Thermus thermophilus driven by the acid-base transition was investigated. The rate of ATP synthesis increased in parallel with the increase in proton motive force (PMF) >110 mV which is composed of a difference in proton concentration (Delta pH) and the electrical potential differences (Delta Psi) across membranes. The optimum rate of synthesis reached 85 s(-1), and the H+/ATP ratio of 4.0 +/- 0.1 was obtained. ATP was synthesized at a considerable rate solely by Delta pH, indicating Delta Psi was not absolutely required for synthesis. Consistent with the H+/ATP ratio, cryoelectron micrograph images of 2D crystals of the membrane-bound rotor ring of the V-ATPase at 7.0-angstrom resolution showed the presence of 12 V-o-c subunits, each composed of two transmembrane helices. These results indicate that symmetry mismatch between the rotor and catalytic domains is not obligatory for rotary ATPases/synthases. |
| DOI | 10.1073/pnas.0706914105 |
| ISSN | 0027-8424 |
| PMID | 18077374 |