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ツゲ ヒデアキ
TSUGE HIDEAKI
津下 英明 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Role of sphingomyelinase in infectious diseases caused by Bacillus cereus |
| 執筆形態 | その他 |
| 掲載誌名 | PLoS ONE |
| 出版社・発行元 | PUBLIC LIBRARY SCIENCE |
| 巻・号・頁 | 7(6) |
| 著者・共著者 | Oda, M.,Hashimoto, M.,Takahashi, M.,Ohmae, Y.,Seike, S.,Kato, R.,Fujita, A.,Tsuge, H.,Nagahama, M.,Ochi, S.,Sasahara, T.,Hayashi, S.,Hirai, Y.,Sakurai, J. |
| 概要 | Bacillus cereus (B. cereus) is a pathogen in opportunistic infections. Here we show that Bacillus cereus sphingomyelinase (Bc-SMase) is a virulence factor for septicemia. Clinical isolates produced large amounts of Bc-SMase, grew in vivo, and caused death among mice, but ATCC strains isolated from soil did not. A transformant of the ATCC strain carrying a recombinant plasmid containing the Bc-SMase gene grew in vivo, but that with the gene for E53A, which has little enzymatic activity, did not. Administration of an anti-Bc-SMase antibody and immunization against Bc-SMase prevented death caused by the clinical isolates, showing that Bc-SMase plays an important role in the diseases caused by B. cereus. Treatment of mouse macrophages with Bc-SMase resulted in a reduction in the generation of H2O2 and phagocytosis of macrophages induced by peptidoglycan (PGN), but no effect on the release of TNF-alpha and little release of LDH under our experimental conditions. Confocal laser microscopy showed that the treatment of mouse macrophages with Bc-SMase resulted in the formation of ceramide-rich domains. A photobleaching analysis suggested that the cells treated with Bc-SMase exhibited a reduction in membrane fluidity. The results suggest that Bc-SMase is essential for the hydrolysis of SM in membranes, leading to a reduction in phagocytosis. |
| DOI | 10.1371/journal.pone.0038054 |
| ISSN | 1932-6203 |
| Put Code(ORCID) | 6110960 |
| PermalinkURL | http://www.scopus.com/inward/record.url?eid=2-s2.0-84862017218&partnerID=MN8TOARS |