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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/07 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Molecular mechanism of energy conservation in polysulfide respiration |
| 執筆形態 | その他 |
| 掲載誌名 | Nature Structural and Molecular Biology |
| 巻・号・頁 | 15(7),pp.730-737 |
| 著者・共著者 | Mika Jormakka,Ken Yokoyama,Takahiro Yano,Masatada Tamakoshi,Satoru Akimoto,Tatsuro Shimamura,Paul Curmi,So Iwata |
| 概要 | Bacterial polysulfide reductase (PsrABC) is an integral membrane protein complex responsible for quinone-coupled reduction of polysulfide, a process important in extreme environments such as deep-sea vents and hot springs. We determined the structure of polysulfide reductase from Thermus thermophilus at 2.4-Å resolution, revealing how the PsrA subunit recognizes and reduces its unique polyanionic substrate. The integral membrane subunit PsrC was characterized using the natural substrate menaquinone-7 and inhibitors, providing a comprehensive representation of a quinone binding site and revealing the presence of a water-filled cavity connecting the quinone binding site on the periplasmic side to the cytoplasm. These results suggest that polysulfide reductase could be a key energy-conserving enzyme of the T. thermophilus respiratory chain, using polysulfide as the terminal electron acceptor and pumping protons across the membrane via a previously unknown mechanism. © 2008 Nature Publishing Group. |
| DOI | 10.1038/nsmb.1434 |
| ISSN | 1545-9993 |
| PMID | 18536726 |