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ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/02 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Cryo-EM Structure of the Native GroEL-GroES Complex from Thermus thermophilus Encapsulating Substrate Inside the Cavity |
| 執筆形態 | その他 |
| 掲載誌名 | STRUCTURE |
| 出版社・発行元 | CELL PRESS |
| 巻・号・頁 | 17(2),pp.287-293 |
| 著者・共著者 | Ryo Kanno,Ayumi Koike-Takeshita,Ken Yokoyama,Hideki Taguchi,Kaoru Mitsuoka |
| 概要 | The chaperonin GroEL interacts with various proteins, leading them to adopt their correct conformations with the aid of GroES and ATP. The actual mechanism is still being debated. In this study, by use of cryo-electron microscopy, we determined the solution structure of the Thermus thermophilus GroEL-GroES complex encapsulating its substrate proteins. We observed the averaged density of substrate proteins in the center of the GroEL-GroES cavity. The position of the averaged substrate density in the cavity suggested a repulsive interaction between a majority of the substrate proteins and the interior wall of the cavity, which is suitable for substrate release. In addition, we observed a distortion of the cis-GroEL ring, especially at the position near the substrate, which indicated that the interaction between the encapsulated proteins and the GroEL ring results in an adjustment in the cavity's shape to accommodate the substrate. |
| DOI | 10.1016/j.str.2008.12.012 |
| ISSN | 0969-2126/1878-4186 |
| PMID | 19217399 |