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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | ERK regulates Golgi and centrosome orientation towards the leading edge through GRASP65 |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Cell Biology |
| 出版社・発行元 | ROCKEFELLER UNIV PRESS |
| 巻・号・頁 | 182(5),pp.837-843 |
| 著者・共著者 | Bisel, B.,Wang, Y.,Wei, J.-H.,Xiang, Y.,Tang, D.,Miron-Mendoza, M.,Yoshimura, S.-I.,Nakamura, N.,Seemann, J. |
| 概要 | Directed cell migration requires the orientation of the Golgi and centrosome toward the leading edge. We show that stimulation of interphase cells with the mitogens epidermal growth factor or lysophosphatidic acid activates the extracellular signal-regulated kinase (ERK), which phosphorylates the Golgi structural protein GRASP65 at serine 277. Expression of a GRASP65 Ser277 to alanine mutant or a GRASP65 1-201 truncation mutant, neither of which can be phosphorylated by ERK, prevents Golgi orientation to the leading edge in a wound assay. We show that phosphorylation of GRASP65 with recombinant ERK leads to the loss of GRASP65 oligomerization and causes Golgi cisternal unstacking. Furthermore, preventing Golgi polarization by expressing mutated GRASP65 inhibits centrosome orientation, which is rescued upon disassembly of the Golgi structure by brefeldin A. We conclude that Golgi remodeling, mediated by phosphorylation of GRASP65 by ERK, is critical for the establishment of cell polarity in migrating cells. |
| DOI | 10.1083/jcb.200805045 |
| ISSN | 0021-9525 |
| Put Code(ORCID) | 19809397 |