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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization |
| 執筆形態 | その他 |
| 掲載誌名 | GENES & DEVELOPMENT |
| 出版社・発行元 | COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT |
| 巻・号・頁 | 23(5),pp.619-632 |
| 担当区分 | 筆頭著者 |
| 著者・共著者 | Yuichiro Mishima,Cei Abreu-Goodger,Alison A. Staton,Carlos Stahlhut,Chong Shou,Chao Cheng,Mark Gerstein,Anton J. Enright,Antonio J. Giraldez |
| 概要 | microRNAs (miRNAs) represent similar to 4% of the genes in vertebrates, where they regulate deadenylation, translation, and decay of the target messenger RNAs (mRNAs). The integrated role of miRNAs to regulate gene expression and cell function remains largely unknown. Therefore, to identify the targets coordinately regulated by muscle miRNAs in vivo, we performed gene expression arrays on muscle cells sorted from wild type, dicer mutants, and single miRNA knockdown embryos. Our analysis reveals that two particular miRNAs, miR-1 and miR-133, influence gene expression patterns in the zebrafish embryo where they account for >54% of the miRNA-mediated regulation in the muscle. We also found that muscle miRNA targets (1) tend to be expressed at low levels in wildtype muscle but are more highly expressed in dicer mutant muscle, and (2) are enriched for actin-related and actin-binding proteins. Loss of dicer function or down-regulation of miR-1 and miR-133 alters muscle gene expression and disrupts actin organization during sarcomere assembly. These results suggest that miR-1 and miR-133 actively shape gene expression patterns in muscle tissue, where they regulate sarcomeric actin organization. |
| DOI | 10.1101/gad.1760209 |
| ISSN | 0890-9369 |
| PMID | 19240126 |