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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Enhanced Inflammation and Accelerated Wound Closure Following Tetraphorbol Ester Application or Full-Thickness Wounding in Mice Lacking Hyaluronan Synthases Has1 and Has3 |
| 執筆形態 | その他 |
| 掲載誌名 | JOURNAL OF INVESTIGATIVE DERMATOLOGY |
| 出版社・発行元 | NATURE PUBLISHING GROUP |
| 巻・号・頁 | 132(1),pp.198-207 |
| 著者・共著者 | Judith A. Mack,Ron J. Feldman,Naoki Itano,Koji Kimata,Mark Lauer,Vincent C. Hascall,Edward V. Maytin |
| 概要 | Hyaluronan (HA) is an abundant matrix molecule, the function of which in the skin remains to be fully defined. To explore the roles of HA in cutaneous injury responses, double-knockout mice (abbreviated as Has1/3 null) that lack two HA synthase enzymes (Has1 and Has3), but still express functional Has2, were used in two types of experiments: (i) application of 12-O-tetradecanoylphorbol-13-acetate (TPA) and (ii) full-thickness wounding of the skin. Uninjured Has1/3-null mice were phenotypically normal. However, after TPA, the accumulation of HA that normally occurs in wild-type epidermis was blunted in Has1/3-null epidermis. In excisional wound-healing experiments, wound closure was significantly faster in Has1/3 null than in wild-type mice. Coincident with this abnormal wound healing, a marked decrease in epidermal and dermal HA and a marked increase in neutrophil efflux from cutaneous blood vessels were observed in Has1/3-null skin relative to wild-type skin. Has1/3-null wounds displayed an earlier onset of myofibroblast differentiation. In summary, selective loss of Has1 and Has3 leads to a proinflammatory milieu that favors recruitment of neutrophils and other inflammation-related changes in the dermis. |
| DOI | 10.1038/jid.2011.248 |
| ISSN | 0022-202X |