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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Insensitivity to glutamate neurotoxicity mediated by NMDA receptors in association with delayed mitochondrial membrane potential disruption in cultured rat cortical neurons |
| 執筆形態 | その他 |
| 掲載誌名 | Journal of Neurochemistry |
| 出版社・発行元 | WILEY-BLACKWELL |
| 巻・号・頁 | 105(5),pp.1886-1900 |
| 著者・共著者 | Kambe, Y.,Nakamichi, N.,Georgiev, D.D.,Nakamura, N.,Taniura, H.,Yoneda, Y. |
| 概要 | We have attempted to elucidate mechanisms underlying differential vulnerability to glutamate (Glu) using cultured neurons prepared from discrete structures of embryonic rat brains. Brief exposure to Glu led to a significant decrease in the mitochondrial activity in hippocampal neurons cultured for 9 or 12 days at 10 mu M to 1 mM with an apoptosis-like profile, without markedly affecting that in cortical neurons. Brief exposure to Glu also increased lactate dehydrogenase release along with a marked decrease in the number of cells immunoreactive for a neuronal marker protein in hippocampal, but not cortical, neurons. Similar insensitivity was seen to the cytotoxicity by NMDA, but not to that by tunicamycin, 2,4-dinitrophenol, hydrogen peroxide or A23187, in cortical neurons. However, NMDA was more efficient in increasing intracellular free Ca(2+) levels in cortical neurons than in hippocampal neurons. Antagonists for neuroprotective metabotropic Glu receptors failed to significantly affect the insensitivity to Glu, while NMDA was more effective in disrupting mitochondrial membrane potentials in hippocampal than cortical neurons. These results suggest that cortical neurons would be insensitive to the apoptotic neurotoxicity mediated by NMDA receptors through a mechanism related to mitochondrial membrane potentials, rather than intracellular free Ca(2+) levels, in the rat brain. |
| DOI | 10.1111/j.1471-4159.2008.05270.x |
| ISSN | 0022-3042 |
| Put Code(ORCID) | 19809401 |