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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009/10 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | DAZL Relieves miRNA-Mediated Repression of Germline mRNAs by Controlling Poly(A) Tail Length in Zebrafish |
| 執筆形態 | その他 |
| 掲載誌名 | PLOS ONE |
| 出版社・発行元 | PUBLIC LIBRARY SCIENCE |
| 巻・号・頁 | 4(10),pp.e7513 |
| 著者・共著者 | Yasuaki Takeda,Yuichiro Mishima,Toshinobu Fujiwara,Hiroshi Sakamoto,Kunio Inoue |
| 概要 | Background: During zebrafish embryogenesis, microRNA (miRNA) miR-430 contributes to restrict Nanos1 and TDRD7 to primordial germ cells (PGCs) by inducing mRNA deadenylation, mRNA degradation, and translational repression of nanos1 and tdrd7 mRNAs in somatic cells. The nanos1 and tdrd7 3'UTRs include cis-acting elements that allow activity in PGCs even in the presence of miRNA-mediated repression.
Methodology/Principal Findings: Using a GFP reporter mRNA that was fused with tdrd7 3'UTR, we show that a germline-specific RNA-binding protein DAZ-like (DAZL) can relieve the miR-430-mediated repression of tdrd7 mRNA by inducing poly(A) tail elongation (polyadenylation) in zebrafish. We also show that DAZL enhances protein synthesis via the 3'UTR of dazl mRNA, another germline mRNA targeted by miR-430. Conclusions/Significance: Our present study indicated that DAZL acts as an "anti-miRNA factor'' during vertebrate germ cell development. Our data also suggested that miRNA-mediated regulation can be modulated on specific target mRNAs through the poly(A) tail control. |
| DOI | 10.1371/journal.pone.0007513 |
| ISSN | 1932-6203 |
| PMID | 19838299 |