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エンドウ トシヤ
ENDO TOSHIYA
遠藤 斗志也 所属 京都産業大学 生命科学部 先端生命科学科 職種 客員教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 1994/04 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | YGE1P, A EUKARYOTIC GRP-E HOMOLOG, IS LOCALIZED IN THE MITOCHONDRIAL MATRIX AND INTERACTS WITH MITOCHONDRIAL HSP70 |
| 執筆形態 | その他 |
| 掲載誌名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS |
| 出版社・発行元 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 巻・号・頁 | 200(1),pp.435-442 |
| 著者・共著者 | M NAKAI,Y KATO,E IKEDA,A TOHE,T ENDO |
| 概要 | Yeast Yge1p, the gene product of YGE1, is a eukaryotic GrpE homolog found recently (Ikeda et al., 1994). We have revealed here that Yge1p is a soluble protein in the mitochondrial matrix. Depletion of Yge1p in yeast cells resulted in accumulation of the precursor of F-1-ATPase a subunit in vivo, suggesting that Yge1p is involved in protein import into mitochondria. Overexpression of Yge1p in the temperature-sensitive mutant strains of mitochondrial hsp70, Ssc1p, caused hypersensitivity to temperature for cell growth, suggesting a genetic interaction between the YGE1 and SSC1 genes. A physical interaction between Yge1p and Ssc1p was directly demonstrated by co-immunoprecipitation of Ssc1p by the anti-Yge1p antibodies. We propose that Yge1p functions in cooperation with Ssc1p in a similar manner as bacterial GrpE with DnaK. (C) 1994 Academic Press, Inc. |
| ISSN | 0006-291X/1090-2104 |