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イタノ ナオキ
ITANO NAOKI
板野 直樹 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/03 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Restriction of Mast Cell Proliferation through Hyaluronan Synthesis by Co-cultured Fibroblasts |
| 執筆形態 | その他 |
| 掲載誌名 | BIOLOGICAL & PHARMACEUTICAL BULLETIN |
| 出版社・発行元 | PHARMACEUTICAL SOC JAPAN |
| 巻・号・頁 | 35(3),pp.408-412 |
| 著者・共著者 | Hirotsugu Takano,Kazuyuki Furuta,Kazuhito Yamashita,Mariko Sakanaka,Naoki Itano,Eiichi Gohda,Kazuhisa Nakayama,Koji Kimata,Yukihiko Sugimoto,Atsushi Ichikawa,Satoshi Tanaka |
| 概要 | Appropriate culture models for tissue mast cells are required to determine how they are involved in regulation of local immune responses. We previously established a culture model for cutaneous mast cells, in which bone marrow-derived immature mast cells were co-cultured with Swiss 3T3 fibroblasts in the presence of stem cell factor. In this study, we focused on the roles of hyaluronan, which is produced by the feeder fibroblasts and forms the extracellular matrix during the co-culture period. Hyaluronan synthesis was found to be mediated by hyaluronan synthase 2 (HAS2) expressed in Swiss 3T3 cells. A decreases in the amount of hyaluronan, which was achieved by retroviral expression of short hairpin RNA for Has2 or by addition of hyaluronidase, significantly enhanced the proliferation of the cultured mast cells without any obvious effects on their maturation. Although we previously demonstrated that CD44 is required for proliferation of cutaneous mast cells, the deficiency of hyaluronan did not affect the proliferation of the cultured mast cells that lack CD44. These findings suggest that the extracellular matrix containing hyaluronan may have a potential to restrict proliferation of cutaneous mast cells in a CD44-independent manner. |
| ISSN | 0918-6158 |