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ミシマ ユウイチロウ
Mishima Yuichiro
三嶋 雄一郎 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2010/06 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | A Novel miRNA Processing Pathway Independent of Dicer Requires Argonaute2 Catalytic Activity |
| 執筆形態 | その他 |
| 掲載誌名 | SCIENCE |
| 出版社・発行元 | AMER ASSOC ADVANCEMENT SCIENCE |
| 巻・号・頁 | 328(5986),pp.1694-1698 |
| 著者・共著者 | Daniel Cifuentes,Huiling Xue,David W. Taylor,Heather Patnode,Yuichiro Mishima,Sihem Cheloufi,Enbo Ma,Shrikant Mane,Gregory J. Hannon,Nathan D. Lawson,Scot A. Wolfe,Antonio J. Giraldez |
| 概要 | Dicer is a central enzyme in microRNA (miRNA) processing. We identified a Dicer-independent miRNA biogenesis pathway that uses Argonaute2 (Ago2) slicer catalytic activity. In contrast to other miRNAs, miR-451 levels were refractory to dicer loss of function but were reduced in MZago2 (maternal-zygotic) mutants. We found that pre-miR-451 processing requires Ago2 catalytic activity in vivo. MZago2 mutants showed delayed erythropoiesis that could be rescued by wild-type Ago2 or miR-451-duplex but not by catalytically dead Ago2. Changing the secondary structure of Dicer-dependent miRNAs to mimic that of pre-miR-451 restored miRNA function and rescued developmental defects in MZdicer mutants, indicating that the pre-miRNA secondary structure determines the processing pathway in vivo. We propose that Ago2-mediated cleavage of pre-miRNAs, followed by uridylation and trimming, generates functional miRNAs independently of Dicer. |
| DOI | 10.1126/science.1190809 |
| ISSN | 0036-8075 |
| PMID | 20448148 |