|
ヨコヤマ ケン
YOKOYAMA KEN
横山 謙 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
|
| 言語種別 | 英語 |
| 発行・発表の年月 | 2012/01 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | MRT letter: Expression of ATP sensor protein in Caenorhabditis elegans |
| 執筆形態 | その他 |
| 掲載誌名 | MICROSCOPY RESEARCH AND TECHNIQUE |
| 出版社・発行元 | WILEY-BLACKWELL |
| 巻・号・頁 | 75(1),pp.15-19 |
| 著者・共著者 | Jun-Ichi Kishikawa,Makoto Fujikawa,Hiromi Imamura,Kayo Yasuda,Hiroyuki Noji,Naoaki Ishii,Shohei Mitani,Ken Yokoyama |
| 概要 | Adenosine 5'-triphosphate (ATP) is the major energy currency and is involved in many biological processes. The ATP-monitoring system for cells in animals can be helpful to study the relationship between energy metabolism and biological processes. The fluorescent ATP biosensor ATeam (ATP indicator based on Epsilon subunit for Analytical Measurements), which has been reported to monitor ATP levels in cultured cells on the basis of fluorescence resonance energy transfer (FRET), was introduced into nematodes by microinjection and UV-irradiation method. To confirm whether ATeam functions as an ATP sensor in nematode cells, the authors measured FRET of ATeam in cells of transgenic nematode. The ATeam was expressed in target cells in nematode. In vulva cells, ATP levels in the cytosol were higher than those in mitochondria. ATeam also sensed ATP level change in cultured cells from the transgenic nematode. These experiments indicated that ATeam is available for detection of changes in ATP levels in nematode cells. Microsc. Res. Tech., 2012. (c) 2011 Wiley Periodicals, Inc. |
| DOI | 10.1002/jemt.21103 |
| ISSN | 1059-910X |
| PMID | 22038755 |