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テラチ トオル
TERACHI TORU
寺地 徹 所属 京都産業大学 生命科学部 産業生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2008/10 |
| 形態種別 | その他 |
| 標題 | Inhibition of chalcone synthase expression in anthers of Raphanus sativus with Ogura male sterile cytoplasm |
| 執筆形態 | その他 |
| 掲載誌名 | ANNALS OF BOTANY |
| 出版社・発行元 | OXFORD UNIV PRESS |
| 巻・号・頁 | 102(4),pp.483-489 |
| 著者・共著者 | Soojung Yang,Toru Terachi,Hiroshi Yamagishi |
| 概要 | Background and Aims Expression of the mitochondrial gene orf138 causes Ogura cytoplasmic male sterility (CMS) in Raphanus sativus, but little is known about the mechanism by which CMS takes place. A preliminary microarray experiment revealed that several nuclear genes concerned with flavonoid biosynthesis were inhibited in the male-sterile phenotype. In particular, a gene for one of the key enzymes for flavonoid biosynthesis, chalcone synthase (CHS), was strongly inhibited. A few reports have suggested that the inhibition of CHS causes nuclear-dependent male sterile expression; however, there do not appear to be any reports elucidating the effect of CHS on CMS expression. In this study, the expression patterns of the early genes in the flavonoid biosynthesis pathway, including CHS, were investigated in normal and male-sterile lines.
Methods In order to determine the aberrant stage for CMS expression, the characteristics of male-sterile anthers are observed using light and transmission electron microscopy for several stages of flower buds. The expression of CHS and the other flavonoid biosynthetic genes in the anthers were compared between normal and male-sterile types using real time RT-PCR. Key Results Among the flavonoid biosynthetic genes analysed, the expression of CHS was strongly inhibited in the later stages of anther development in sterility cytoplasm; accumulation of putative naringenin derivatives was also inhibited. Conclusions These results show that flavonoids play an important role in the development of functional pollen, not only in nuclear-dependent male sterility, but also in CMS. |
| DOI | 10.1093/aob/mcn116 |
| ISSN | 0305-7364 |