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ナカムラ ノブヒロ
NAKAMURA NOBUHIRO
中村 暢宏 所属 京都産業大学 生命科学部 先端生命科学科 職種 教授 |
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| 言語種別 | 英語 |
| 発行・発表の年月 | 2009 |
| 形態種別 | 研究論文 |
| 査読 | 査読あり |
| 標題 | Interference by adrenaline with chondrogenic differentiation through suppression of gene transactivation mediated by Sox9 family members |
| 執筆形態 | その他 |
| 掲載誌名 | Bone |
| 出版社・発行元 | ELSEVIER SCIENCE INC |
| 巻・号・頁 | 45(3),pp.568-578 |
| 著者・共著者 | Takarada, T.,Hojo, H.,Iemata, M.,Sahara, K.,Kodama, A.,Nakamura, N.,Hinoi, E.,Yoneda, Y. |
| 概要 | In contrast to osteoblasts, little attention has been paid to the functional expression of adrenergic signaling machineries in chondrocytes. Expression of mRNA was for the first time demonstrated for different adrenergic receptor (AdR) subtypes in chondrogenic ATDC5 cells and mouse metatarsals isolated before vascularization in culture, but not for other molecules related to adrenergic signaling. In neonatal mouse tibial sections, beta(2)AdR and alpha(2a)AdR mRNA expression was found in chondrocytes at different developmental stages by in situ hybridization. Exposure to adrenaline significantly suppressed expression of several maturation markers through the cAMP/protein kinase A pathway activated by beta(2)AdR without affecting cellular proliferation in both Cultured ATDC5 cells and metatarsals. Adrenaline also significantly inhibited gene transactivation by sry-type HMG box 9 (Sox9) family members essential for chondrogenic differentiation in a manner prevented by the general beta AdR antagonist propranolol, with a concomitant significant decrease in the levels of Sox6 mRNA and corresponding protein, in ATDC5 cells and primary cultured Mouse costal chondrocytes. Systemic administration of propranolol significantly promoted the increased expression of mRNA for collagen I and collagen X, but not for collagen 11, in callus of fractured femur in mice. These results suggest that adrenaline may interfere with chondrogenic differentiation through downregulation of Sox6 expression for subsequent suppression of gene transactivation mediated by Sox9 family members after activation Of beta(2)AdR expressed by chondrocytes. (C) 2009 Elsevier Inc. All rights reserved. |
| DOI | 10.1016/j.bone.2009.05.004 |
| ISSN | 8756-3282 |
| Put Code(ORCID) | 19809392 |